• Differential Responses of Phytophthora sojae to Seed Exudates of Host Soybean and Non-host Maize

    Wen Jing-zhi;Zhang Zhuo-qun;Xu Ying;Song Guang-mei;Chen Yu-fei;Gao Xin-ying;Zhao Yu-qi;Jia Meng-zhen;Liang Jia-yu;

    In order to clarify the differential response of Phytophthora sojae to the seed exudates of host soybean and non-host maize and understand the relationship between seed exudates and host selectivity of Phytophthora sojae, non-host maize Suiyu 23 and susceptible host soybean Sloan seed exudates were collected to measure their influence on mycelial growth, formation and germination of oospores, chemotaxis, encystment and germination of zoospores of Phytophthora sojae. The results showed that nonhost maize seed exudates exhibited repellency to zoospores of Phytophthora sojae, it also could significantly inhibited Phytophthora sojae mycelial growth, formation of oospores compared with the control; compared with host soybean, non-host maize seed exudates could significantly inhibited Phytophthora sojae mycelial growth, formation and germination of oospores, germination of cysts, which indicated that the seed exudates was the critical factor to host selectivity of Phytophthora sojae and the maize seed exudates was closely related to its non-host resistance.

    2019年03期 v.26;No.76 1-8页 [查看摘要][在线阅读][下载 1085K]
    [下载次数:19 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Identification of QTL and Analysis QTL with Tolerance to Sclerotinia sclerotiorum in Soybean

    Zhao Xue;Jiang Hai-peng;Song Wei;Feng Lei;Tan Jia-qi;Han Ying-peng;Li Wen-bin;

    Soybean white mold(SWM) caused by Sclerotinia sclerotiorum is a serious disease of soybean and other plant, which is mainly distributed in the soybean producing areas of north China, east China, southwest and northeast China. The tolerance of soybean to sclerotium is partial resistance(quantitative trait), which is controlled by multiple genes. Mapping QTL and identifying candidate genes underlying soybean tolerance to SWM can accelerate the process of breeding for disease-resistant varieties. In the present study, a total of 128 lines derived from the susceptible soybean cultivar Hefeng25 and the disease tolerant soybean cultivar Maple Arrow were evaluated by in vitro and in vivo inoculation methods. A total of 78 SSR markers were used to construct linkage groups(D1 a(Chr.01), A2(Chr.08), B1(Chr.11) and F(Chr.13)) which intensively distributed SWM resistance related QTLs. Five QTLs were detected through combining two sets of phenotypic data with the composite interval mapping(CIM) method. A total of seven candidate genes located in the five QTLs were induced by Sclerotinia sclerotiorum. The SSR markers and candidate genes associated with tolerance to Sclerotinia sclerotiorum could be helpful for SWM resistance breeding in soybean.

    2019年03期 v.26;No.76 9-17页 [查看摘要][在线阅读][下载 614K]
    [下载次数:21 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:11 ]
  • Screening of Transgenic Soybean Materials for Salt Tolerance

    Yang Yu-ying;Deng Jing;Ma Qiao-cong;Zhang Wei-na;Zhang Bin-bin;

    Soybean is an important oil crop in China, which plays critical roles in China grain market. Therefore, researches on salttolerant soybean cultivars are of great significance and important for the sustainable development and utilization of the agricultural land resources. In this study, transgenic GmIPK-II(IPK-II) soybean seedlings from three single lines and 10 GmHAL-I(HAL-I) transgenic lines were provided by Jilin Academy of Agricultural Sciences. Wild type seedlings of soybean cultivars Dongnong 50(DN50) and Huachun 6(HC6) were the controls. Transgenic and wild-type seedlings were evaluated under different salt concentrations. Moreover, three kinds of culture methods and two kinds of salt application methods including "irrigated from top" and "permeated from bottom" were discussed. Through observing the changes of phenotype from transgenic and control seedlings under different salt concentrations, the salt tolerance screening evaluation system with high efficiency was initially confirmed, which conditions were as the followings: vermiculite and turfy soil were mixed as 1 : 1. Concentration of NaCl was 150 mmol · L~(-1). Time of the treatments was 6 days. Finally, 23 transgenic lines of GmIPK-II and GmHAL-I with higher salt-tolerance were obtained, which laid a foundation for researches.

    2019年03期 v.26;No.76 18-26页 [查看摘要][在线阅读][下载 2543K]
    [下载次数:8 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:8 ]
  • Development, Reproduction and Body Size Variation of Aphis glycines Matsumura Fed on Different Plants

    Liu Jian;Wang Su-ji;Bai Bing;Gao Bo;Fan Yan-jie;

    Soybean aphid, Aphis glycines Matsumura is one of the most important pests in soybean. Life cycle of A. glycines is characterized as heteroecious and holocyclic. The primary hosts of A. glycines are Rhamnus spp. and the secondary hosts include cultivated soybean, Glycine max(L.) Merr, and wild soybean, Glycine soja Sieb. & Zucc. In this study, A. glycines were fed on Trifolium repens L. and Metaplexis japonica(Thunb.) Makino for three generations and their development, reproduction and body sizes were studied. These data were compared to the control fed on the known hosts, G. max and G. soja. These newly deposited offspring by the 3 rd generation were transferred back onto G. max and these differences in their development, reproduction and body sizes were also studied. It showed that A. glycines all could survive, develop and reproduce well, when they were fed on T. repens and M. japonica for three generations, but there were significant differences in their nymph stage, adult longevity and fecundity, life table parameters and body sizes. When these offspring were transferred back onto G. max which were newly deposited by the 3 rd generation aphids fed on T. repens and M. japonica, they could survive, develop and reproduce well, but there were significant differences in their nymph stage, adult longevity, intrinsic rate of increase and body sizes. It provided important information for studies on host adaptability of A. glycines on T. repens and M. japonica and to make clear the potential that this aphid involved into host biotypes on these plants.

    2019年03期 v.26;No.76 27-35页 [查看摘要][在线阅读][下载 424K]
    [下载次数:10 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:4 ]
  • Effects of Bacillus subtilis on Degradation of Cellulose

    Zhou Chun-shuang;Wang Zhe;Li Zhi-yuang;Wen Xue-peng;Gao Xue-jun;

    Bacillus subtilis is a representative probiotic widely used in food, medicine, livestock and other industries. In this experiment a strain of Bacillus subtilis was isolated and identified, and its ability to degrade cellulose was also measured. The results showed that Bacillus subtilis had strong capacity to degrade cellulose(62.3% was degraded) and weak capacity to degrade hemicellulose(17.2% was degraded), while it could hardly degrade lignin. The total protein amount in the fermentation medium with cellulose-rich substrate reached by 9.4% after Bacillus subtilis fermentation, compared with that without cellulose-rich substrate. Furthermore, the amounts of Met, Lys and Leu reached by 31.4%, 42.2% and 4.9%, respectively. At 36 h of fermentation, the activity of cellulase reached the highest, and at this time the activity of the enzyme was obtained at 7.19 U · mL~(-1). The mRNA expression level of the cellulase gene was detected by qPCR, and the experimental group with cellulose substrate was about 2.5 times more than that of the non-cellulose substrate control group. These above results demonstrated that this strain of Bacillus subtilis had a strong ability to degrade cellulose, and synthesize more proteins and restrictive essential amino acids. This study revealed that Bacillus subtilis was a new alternative to ferment cellulose substrates to produce commercial feed or feed additives.

    2019年03期 v.26;No.76 36-45页 [查看摘要][在线阅读][下载 533K]
    [下载次数:5 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:7 ]
  • Effects of Dietary Fat Levels on Growth, Nutrient Digestibility, Nitrogen Utilization and Fur Quality of Growing-furring Blue Foxes

    Cao Shu-xin;Liu Da-wei;Wang Chao;Wei Jing-kun;Sun Jia-you;Bai Yang;Xu Liang-mei;

    This study evaluated the effects of dietary fat levels on the growth, nutrient digestibility, nitrogen balance and pelt characteristics of the blue fox(Alopex lagopus). Age-matched blue foxes(12 weeks old) with similar body weights were randomly divided into six dietary groups over 98 days, during the growing-furring period. Blue foxes in the six groups were fed with experimental diets containing 10%(groupⅠ), 12%(groupⅡ), 14%(group Ⅲ), 16%(group Ⅳ), 18%(groupⅤ) and 20%(group Ⅵ) fat during the growing period(from 13 weeks to 18 weeks of age); foxes were fed with diets containing 12%(groupⅠ), 14%(groupⅡ), 16%(group Ⅲ), 18%(group Ⅳ), 20%(groupⅤ) and 22%(group Ⅵ) fat during the furring period(from 18 weeks to 26 weeks of age). The results indicated that 16%-18% dietary fat, during the growing period significantly improved nutrient utilization, and as a main energy source, significantly reduced the feed/gain ratio. A dietary fat level of 18%, during the furring period not only improved the growth and development and decreased the feed/gain ratio, but also increased nutrient digestibility and fur quality. These data, combined with data from a conic regression model, showed that dietary fat levels between 16.29% and 18.40%, during the growing period and between 17.72% and 18.72%, during the furring period enhanced the growth performance and fur quality of blue foxes.

    2019年03期 v.26;No.76 46-56页 [查看摘要][在线阅读][下载 422K]
    [下载次数:5 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Phylogeny and Homologous Recombination Occurring in Classical Swine Fever Viruses

    Jiang Qing-yun;Gao Yu;Li Wei-qun;Ding Fan;Juergen Richt;Ren Yu-dong;Li Guang-xing;

    Classical swine fever virus(CSFV) is the causative agent of classical swine fever, a highly contagious disease of pigs. But there is little information on the recombination in natural populations of CSFVs. Therefore, a phylogenetic analysis of 62 fulllength genome CSFV strains, isolated from all over the world, was performed to detect potential recombination events, with the recombinant sequences being analyzed with the SimPlot and RDP programs. The results identified a mosaic virus, Chinese CSFV HCLV(2)(AF091507.1), which is the one naturally emerged recombinant CSFV with two recombination breakpoints at 2 484 and 2 900 bp of the genome alignment. Its two putative parental-like strains were CSFV Shimen(AF092448.2) and CSFV strain C/HVRI(AY805221.1). This work demonstrated that homologous recombination did occur in natural CSFV populations. It had significant implications for understanding the molecular epidemiology of CSFV, and revealed that recombination was an important factor for high genetic diversities of CSFV.

    2019年03期 v.26;No.76 57-65页 [查看摘要][在线阅读][下载 684K]
    [下载次数:15 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:7 ]
  • Treatment Effect of Fermentation of Ganoderma lucidum to Immune and Antioxidant Functions in Immunosuppressed Mice

    Han Yu-wen;Jiang Xiao-wen;Li Shu-hong;Yu Wen-hui;

    The immune regulatory and antioxidant roles of Ganoderma lucidum were investigated using cyclophosphamide(CTX)-induced immunosuppressed mice. Mice were randomly divided into groups: untreated(groupⅠ), immunosuppressed(groupⅡ), unfermented G. lucidum polysaccharide(groupⅢ) and fermented G. lucidum polysaccharide(group Ⅳ). After seven consecutive days of treatments, the serum concentration of IL-4, IFN-gamma, IgG, IgA and IgM and the liver activity of GSH-Px, SOD, CAT and MDA enzymes were analyzed. The contents of IL-4, IFN-γ in serum and GSH-Px, SOD and CAT in liver tissues were significantly reduced in groupⅡ compared with those in group I, indicating successful CTX-induced immunosuppression. Interestingly, the results showed that the above immune and antioxidant indicators were significantly improved after G. lucidum polysaccharide treatment, regardless of fermentation. However, fermentation caused changes in polysaccharide structure, which might have a significant impact on immune regulation and antioxidant functions in immunosuppressed mice.

    2019年03期 v.26;No.76 66-74页 [查看摘要][在线阅读][下载 567K]
    [下载次数:20 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:5 ]
  • Research on Rice Leaf Disease Recognition Based on BP Neural Network

    Shen Wei-zheng;Guan Ying;Wang Yan;Jing Dong-jun;

    To solve the problem of mistake recognition among rice diseases, automatic recognition methods based on BP(back propagation) neural network were studied in this paper for blast, sheath blight and bacterial blight. Chose mobile terminal equipment as image collecting tool and built database of rice leaf images with diseases under threshold segmentation method. Characteristic parameters were extracted from color, shape and texture. Furthermore, parameters were optimized using the single-factor variance analysis and the effects of BP neural network model. The optimization would simplify BP neural network model without reducing the recognition accuracy. The finally model could successfully recognize 98%, 96% and 98% of rice blast, sheath blight and white leaf blight, respectively.

    2019年03期 v.26;No.76 75-86页 [查看摘要][在线阅读][下载 975K]
    [下载次数:23 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:8 ]
  • Research and Simulation of Navigation Control System for Agricultural Unmanned Rotation Rotorcraft

    Ma Chen-ming;Su Zhong-bin;Zhang Zong-xin;

    In order to improve the efficiency of field operations, such as pesticide spraying and pest control, the development of new agricultural machinery has become the main goal of agricultural modernization. At present, small unmanned aerial vehicles(UAVs) have achieved good results in small area pesticide spraying, but they still do not meet the requirements of the big field operations. The rotation rotorcraft has the characteristics of low speed, good safety, super stol, strong endurance, strong wind resistance and simple mechanism, so it is very suitable for farmland operation. But at present, there are relatively few researches on the unmanned rotation rotorcraft at domestic and abroad, and there is no mature control theory system of unmanned rotation rotorcraft. Therefore, in order to solve the problem of navigation control system of unmanned rotation rotorcraft, the navigation control strategy of the rotation rotorcraft was studied in this paper, and the flight control strategy of the rotation rotorcraft was divided into two parts, the linear control and the turn control. The lateral correction strategy was used to control the rotorcraft, so as to complete the track guidance of the rotation rotorcraft. Using the matrix laboratory(MATLAB) modeling to simulate the circular trajectory and the serpentine trajectory, the maximum deviation of circular path offset was 1.1 m. The variance between the path angle and the given path angle was 6°. The maximum deviation of serpentine path offset was 0.6 m. The variance between the path angle and the given path angle was 8°. The simulation results showed that the rotorcraft could accomplish the scheduled trajectory task based on this control strategy.

    2019年03期 v.26;No.76 87-96页 [查看摘要][在线阅读][下载 760K]
    [下载次数:8 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Journal of Northeast Agricultural University(English Edition) Instruction to Authors

    <正>Aims and Scope Journal of Northeast Agrictultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.

    2019年03期 v.26;No.76 97页 [查看摘要][在线阅读][下载 650K]
    [下载次数:3 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:8 ]
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