- Zhang Junhua;Lv Anqi;Chen Wanying;Liu Qingran;Xiong Tianliang;Zhang Yifei;Tian Ziyu;Wu Jiaju;
In recent years,the prevalence of rice sheath blight caused by Rhizoctonia solani has significantly increased in Heilongjiang Province.Chemical control has become the primary control method.To cope with this,a novel mycelium growth rate method was employed to assess the toxicity of 13 fungicides,including a combination of 45% prochloraz and 125 g·mL~(-1 )epoxiconazole,against R.solani.Additionally,the resistance of 99 R.solani strains to thifluzamide across various regions was also evaluated.The findings indicated that 75% trifloxystrobin-tebuconazole exhibited the most effective inhibitory effect,with an effective inhibitory medium concentration (EC_(50)) value of 0.0101 μg·mL~(-1).The EC_(50) values for 20% prothioconazole,125 g·mL~(-1 ) epoxiconazole,24% thifluzamide,and 50% hexaconazole were all less than 10 μg·mL~(-1),indicating a better inhibitory effect on R.solani.The strongest synergistic effect was noted in the mixture of prochloraz and epoxiconazole at a 1:2 ratio,resulting in an EC_(50) value of 2.9917 μg·mL~(-1),and a co-toxicity coefficient of 213.38.Among the 34 strains from Harbin City,the average EC_(50) value was 196.9341 μg·mL~(-1) indicating the highest susceptiblility to thifluzamide.Conversely,15 strains from Shuangyashan City exhibited an average EC_(50) value of 364.7323 μg·mL~(-1),reflecting the lowest sensitivity to thifluzamide.The sensitivity baseline EC_(50) value for R.solani was 253.8854 μg·mL~(-1),with an overall resistance level between 0.1567 and 3.3292,indicating that the resistance level of R.solani in Heilongjiang Province remained low.Therefore,R.solani was still sensitive to thifluzamide in most areas of Heilongjiang Province,but there was a certain risk of resistance in Qitaihe City,which needed to be continuously monitored.At the same time,this study might provide a theoretical foundation for enhancing the prevention and management of the rice sheath blight.
2025年02期 v.32;No.99 1-9页 [查看摘要][在线阅读][下载 948K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Zhao Jiaqi;Zhang Jiake;Xie Tongyin;
This paper conducted a systematic survey and zoogeographical region analysis of the family Sphingidae in the Guokui Mountain,Heilongjiang Province.Collections were made from May 2023 to August 2024 using the light-trap method.A total of 14 species and 11 subspecies from 18 genera and three subfamilies were recorded.One species (Ambulyx tobii) and two subspecies (Ambulyx japonica koreana and Clanis undulosa undulosa) were new records for Heilongjiang Province.The study showed that the subfamily Smerinthinae had the most species (subspecies),while the subfamily Sphinginae had the fewest.Among the world's zoogeographical region,most species (subspecies) in the Guokui Mountain belonged to the palearctic region,with eight species and seven subspecies were also found in the oriental region.This indicated a close biogeographic connection between the two regions.Among the Chinese zoogeographical regions,the northeastern territory,northern territory and northwestern territory had the most abundant species (subspecies).It was also found that the distribution pattern types of hawkmoths in the Guokui Mountain were diverse,with the 'northeastern territory-northern territory-northwestern territory' and 'northeastern territory-northern territory-northwestern territory-western plateau-southwestern territory-central territory-southeastern territory' types having the most species (subspecies).In addition,the Guokui Mountain hawkmoths were mostly distributed interregionally.The distribution patterns that contained the northeastern territory were the most numerous,followed by the northern territory.The rich diversity of the family Sphingidae in the Guokui Mountain was closely related to the unique climate,environment and vegetation types in the area.The results could help to improve the biodiversity database of Heilongjiang Province and researches on hawkmoths.
2025年02期 v.32;No.99 10-22页 [查看摘要][在线阅读][下载 914K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Dai Jianjun;Liu Zhaoting;Su Dezhen;Wang Jingyi;
In order to explore the remediation effects of lignite and biochar on Zn-contaminated soil,this experiment studied the impacts of adding lignite and biochar on soil respiration,soil enzyme activity,and organic carbon in Zn-contaminated soil through soil culture experiments,which provided a theoretical basis for the remediation and improvement as well as for the development and utilization of Zn-contaminated soil.The study was an L8 (4×2~2) orthogonal experimental design with eight treatments,in which there were four levels of Zn contamination concentration (Z0:0;Z1:125 mg ·kg~(-1);Z2:250 mg ·kg~(-1);Z3:500 mg ·kg~(-1)),low-Zn (125-250 mg ·kg~(-1)) and high-Zn (500 mg ·kg~(-1)),two levels of lignite (H0:0;H1:13.33 g ·kg~(-1)),two levels of biochar (C0:0;C1:3.33 g ·kg~(-1)),with four replicates per treatment.The results showed that lignite or biochar and their interaction had extremely significant effects on both respiration rate and accumulation in Zn-contaminated soil.Among the high Zn-contaminated treatments,the mixed application of lignite and biochar (Z3H1C1 treatment) had the fastest soil respiration rate and the highest soil respiration accumulation.Lignite,biochar and their interaction had significant or extremely significant effects on sucrase,catalase and polyphenol oxidase activities in Zn-contaminated soil.Among the high Zn-contaminated treatments (Z3),the addition of biochar alone had the most significant effects on the increase of soil sucrase and catalase enzyme activities,while the mixed application of lignite and biochar had the most significant effects on the increase of soil polyphenol oxidase activity.Lignite,biochar and their interaction had significant or extremely significant effects on the total organic carbon,active organic carbon and microbial carbon content of Zn-contaminated soils.Soil total organic carbon content in general peaked at day 80.Among the high Zn-contaminated treatments,the addition of biochar alone had the most significant effects on the total organic carbon content of the soil,while the mixed application of lignite and biochar had the most significant effect on the microbiomass carbon content.
2025年02期 v.32;No.99 23-36页 [查看摘要][在线阅读][下载 1198K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Zhang Yixin;Sun Qiaochu;Luo Tianning;Zhang Huayu;Wang Fucheng;Zhang Wen;
The trace element selenium (Se) occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide (LPS) induced mouse mastitis model was established.Mice were fed with moderate Se diet (0.087 mg ·kg~(-1) Se),adequate Se diet (0.15 mg ·kg~(-1) Se) or excessive Se diet (1.5 mg ·kg~(-1) Se) for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha (TNF–α),interleukin–1β (IL–1β) and cyclooxygenase–2 (COX–2);and enhanced the phosphorylation of NF–κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.
2025年02期 v.32;No.99 37-46页 [查看摘要][在线阅读][下载 844K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Liu Junhong;Ying Linlin;Zhou Shuaishuai;Fu Yuxin;Mao Yanjun;Li Yao;
The purpose of this experiment was to investigate the effect and mechanism of quercetin on reproductive performance in perinatal sows.According to the similar principle of parity and body weight,24 perinatal sows were randomly divided into four treatments with six replicates,including normal control and three treatments fed by a basal diet supplemented with 0,0.025%,0.050%,and 0.075% quercetin,respectively.The feeding trial was conducted from 100 days of gestation to 28 days post-partum.Reproductive performance,colostrum composition,serum indexes of antioxidation,inflammation,hormones,and the diversity and relative abundance of fecal microflora were determined in perinatal sows.Compared with the control,quercetin significantly reduced the rate of constipation,serum content of malondialdehyde (MDA),prolactin (PRL),and progesterone (PROG),and significantly increased the content of serum estradiol (E2) and insulin-like growth factor-1 (IGF-1) in colostrum of perinatal sows (P<0.05).Meanwhile,0.025% quercetin significantly increased glutathione (GSH) content in serum (P<0.05),0.050% quercetin significantly reduced the rate of backfat loss (P<0.05),and 0.075% quercetin significantly increased immunoglobulin M (IgM) content in colostrum of perinatal sows (P<0.05).In addition,0.050% and 0.075% quercetin significantly shortened estrus interval (P<0.05) and significantly increased the content of IgA and IgG in colostrum (P<0.05) and dopamine (DA) content in serum of perinatal sows (P<0.05).Quercetin significantly decreased the content of nitric oxide (NO),IL-1β,IL-6,TNF-α,and MCP-1 in serum (P<0.05) and significantly improved microflora abundance and diversity in feces of perinatal sows (P<0.05).In conclusion,dietary supplementation of quercetin reduced oxidative stress and inflammatory responses and regulated intestinal microflora in perinatal sows,thus improved reproductive performance in perinatal sows.Under this experimental condition,the optimal supplementation with dietary quercetin was 0.075% in perinatal sows.
2025年02期 v.32;No.99 47-65页 [查看摘要][在线阅读][下载 1120K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Xu Hongzhao;Zhao Qinghe;Liu Huaxi;Zhang Zifang;Fang Junlong;
Taoren and Xingren are commonly used herbs in East Asian medicine with different medication functions but huge economic differences,and there are cases of adulterated sales in market transactions.An effective adulteration recognition based on hyperspectral technology and machine learning was designed as a non-destructive testing method in this paper.A hyperspectral dataset comprising 500 Taoren and 500 Xingren samples was established;six feature selection methods were considered in the modeling of radial basis function-support vector machine (RBF-SVM),whose interaction between the two optimization methods was further researched.Two mixed metaheuristics modeling methods,Mixed-PSO and Mixed-SA,were designed,which fused both band selection and hyperparameter optimization from two-stage into one with detailed process analysis.The metrics of this mixed model were improved by comparing with traditional two-stage method.The accuracy of Mixed-PSO was 89.2% in five-floods cross-validation that increased 4.818% than vanilla RBF-SVM;the accuracy of Mixed-SA was 88.7% which could reach the same as the traditional two-stage method,but it only relied on 48 crux bands in full 100 bands in RBF-SVM model fitting.
2025年02期 v.32;No.99 66-81页 [查看摘要][在线阅读][下载 2394K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] - Ning Hailong;Wang Yidi;Guan Fangshuo;HuBo;Zhang Shuzhen;Xu Pengfei;Li Wenxia;
Soybean frogeye leaf spot (FLS) disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed quality of soybean.The current resistant varieties are difficult to meet the production demand.The breeders have identified 50 different physiological small species and discussed the physiological and biochemical characteristics of soybean resistance to FLS.In soybean disease resistance breeding,resistance resources are screened for the main physiological races in different countries,resistance materials are created,more than 100 genome regions associated with resistance are located,and 12 resistance-related genes are identified.In order to promote the research of soybean disease resistance breeding,this paper expounded and analyzed the pathogenesis characteristics of soybean FLS,the division of races,the physiological and biochemical mechanism of soybean resistance to FLS disease,quantitative trait locus (QTL),quantitative trait nucleotides (QTN),genes of resistance sites,the screening of resistant germplasm resources,and the breeding of new varieties,so as to gain an in-depth understanding of the pathogenesis principle of soybean FLS disease.In order to provide a theoretical basis and technical support for the breeding of soybean FLS disease,the resistance mechanism of soybean FLS disease was analyzed from the molecular level.
2025年02期 v.32;No.99 82-96页 [查看摘要][在线阅读][下载 1002K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] <正>Instruction to AuthorsAims and Scope Journal of Northeast Agricultural University(English Edition) is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide. It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science, horticulture, plant protection, resource and environment, animal science, veterinary medicine, agricultural engineering and technology, agricultural water conservancy, life science, biotechnology and food science.
2025年02期 v.32;No.99 97页 [查看摘要][在线阅读][下载 333K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ]