- Zhang Junhua;Ren Rui;Yang Xin;Tian Ziyu;Zhao Guohui;Han Songhan;Zhang Yifei;Wu Jiaju;Xu Xiaofeng;Ni Zhe;Yang Mingxiu;
To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province, a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the diseased samples collected from five Chinese herbal medicine planting areas in Heilongjiang Province between the years of 2021 and 2022. Repetitive extragenic palindromic polymerase chain reaction(Rep–PCR) was used to amplify 57 isolates of gray leaf spot pathogen on D. dasycarpus from different regions of Heilongjiang Province. The polymorphic bands amplified by three sets of primers accounted for more than 80%. Cluster analysis results showed that at a similarity coefficient of 0.67, the gray leaf spot pathogen on D. dasycarpus in Heilongjiang Province could be divided into five major genetic groups. Genetic diversity parameter analysis indicated that there were certain differences in genetic richness among the geographic populations of gray leaf spot pathogen on D. dasycarpus from different regions. Analysis of molecular variance(AMOVA) revealed that genetic variation among strains mainly originated within populations. The genetic differentiation and relationships of gray leaf spot pathogen on D. dasycarpus from different geographic regions of Heilongjiang Province indicated that genetic differentiation and kinship among populations were somewhat related to their geographic distance. The greater the geographic distance, the higher the genetic differentiation coefficient, and the lower the genetic uniformity among populations.
2026年01期 v.33;No.102 1-10页 [查看摘要][在线阅读][下载 669K] [下载次数：4 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：8 ] - Liu Wenli;Li Caiwen;Su Xiaomeng;Sun Mujun;Gao Yuetian;Ji Mingshan;
To investigate the eco-economic thresholds of weeds and the critical period for their control,combining economic and ecological perspectives to achieve scientific weed management,four dominant weeds,Echinochloa crus-galli(L.) P.Beauv,Chenopodium album L.,Digitaria sanguinalis(L.) Scop,and Commelina communis L.,were selected as experimental subjects,based on their common occurrence in spring maize planting areas in Northern China.A predictive model for maize yield loss caused by mixed weed populations was established.The study analyzed the eco-economic thresholds of weeds under different control measures and determined the optimal period for weed control by combining the critical control period.A logarithmic function model was developed to describe the relationship between mixed weed density and maize yield loss:y=5.98751n(x)-6.5407(R~2=0.949,F=131.244,P=0.000).The optimal model for the critical period of competition between weeds and maize in maize fields was:y=-0.0027x~2+0.5624x-10.064(R~2=0.968,F=30.513,P=0.032).When the weed density in maize fields reached 5.57 plants·m~(-2),manual weeding should be conducted promptly.When the weed density was 3.41 plants·m~(-2) or 3.48 plants·m~(-2),soil or foliar treatments should be applied,respectively.If the weed density reached 3.93 plants·m~(-2),a combination of soil and foliar treatment should be implemented.The critical period for manual weeding was 28.4 days after sowing,for soil treatment it was 19.9 days,for foliar treatment it was 21.8 days,and for the combined treatment of soil and foliar methods,it was 23.5 days after sowing.Retaining weeds for up to 15 days after maize sowing did not result in a yield loss and could even have a positive effect on maize yield.
2026年01期 v.33;No.102 11-21页 [查看摘要][在线阅读][下载 575K] [下载次数：5 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：2 ] - Di Haoyang;Hou Xinyang;Li Yan;You Xue;Yu Yuxin;Wang Jingang;Zhang Haizhen;
Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure included α-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca~(2+) binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK) activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA) stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.
2026年01期 v.33;No.102 22-34页 [查看摘要][在线阅读][下载 1905K] [下载次数：2 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：1 ] - Shan Mingxu;Wu Zhibo;Liu Guangtao;Li Feng;Yang Chunxu;Xie Yingjie;Zou Sen;Li Xiaoru;Dong Na;
In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E. coli) K88, microorganisms were screened from the feces of Min pigs, and their antibacterial and biological characteristics were studied. Using E. coli K88 as the indicator bacterium, the supernatant of the isolated strain from Min pigs was co-cultured with the indicator bacteria for the antibacterial test. The Bacillus velezensis(B. velezensis) strain MZ-78 showed stronger antimicrobial activity against E. coli K88 than other isolates, and its supernatant inhibited the growth of other pathogens including Salmonella typhimurium(S. typhimurium), E. coli, Staphylococcus aureus(S. aureus), and Pseudomonas aeruginosa(P. aeruginosa). Further studies showed that the selected strain MZ-78 exhibited a certain tolerance to sodium chloride conditions. The B. velezensis strain MZ-78 exhibited no hemolytic activity and was not susceptible to antibiotics. Additionally, during metabolism, it generated a certain quantity of short-chain fatty acids, including isobutyric acid and isovaleric acid. Furthermore, this strain demonstrated the capability to counteract the adhesion of E. coli K88 to porcine small intestinal epithelial cells(IPEC-J2), while also promoting their growth and proliferation. The B. velezensis strain MZ-78 isolated from Min pigs feces showed a series of probiotic characteristics and the potential to survive in the digestive tract of animals, which indicated its potential commercial application value as a probiotic alternative to antibiotics in animal husbandry.
2026年01期 v.33;No.102 35-48页 [查看摘要][在线阅读][下载 958K] [下载次数：3 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：1 ] - Zhou Jiaxin;Liang Hongsheng;Nie Chenyi;Li Kuo;Shang Ruiqiang;Liu Hefei;Yu Zhao;Hu Renjie;Gao Aili;
This study investigated the expression characteristics and clinical significance of gamma-aminobutyric acid A(GABAA) receptor subunit gamma1(GABRG1) in glioma by integrating cancer and normal tissue data from multiple databases and combining them with experimental evidence. The research revealed that GABRG1 exhibited low expression levels in glioma, and its low expression was closely associated with unfavorable clinical outcomes. Furthermore, a prognostic model with strong decision-making ability was constructed using multivariate Cox regression analysis, incorporating age, isocitric dehydrogenase(IDH) mutation status, O6-methylguanine-DNA methyltransferase(MGMT) methylation status, World Health Organization(WHO) grade, and GABRG1 expression. Additionally, GABRG1 might be associated with the expression of tumor-associated macrophages and was significantly linked to immune regulatory processes. This study provided a new target for immunotherapy in glioma and offered a novel indicator for predicting and evaluating the clinical prognosis of patients.
2026年01期 v.33;No.102 49-70页 [查看摘要][在线阅读][下载 3373K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：2 ] - Zheng Xianzhe;Jiang Zhenze;Xue Liangliang;Zhang Yuhan;Bai Caiyu;Liu Huiran;Cong Hongyue;
A machine learning-based APP may quickly and non-destructively evaluate the quality of parameters, such as hardness and anthocyanin content in blue honeysuckle berries(Lonicera caerulea L., BHB), based on changes in pericarp color characteristics. The color feature information of the BHB pericarp was extracted, and the corresponding hardness and anthocyanin content were determined at various growing stages. Correlation analysis of BHB quality indexes was conducted by single and combined components of BHB epidermal color features. The results showed that fruit hardness had a significantly negative correlation with color feature parameter R-G, and its anthocyanin content had a significantly positive correlation with color feature parameter R. Comparing the eight models, random forest(RF) was established to evaluate the hardness and anthocyanin content of BHB according to the correlation between pericarp color features and hardness and anthocyanin content on BHB quality evaluation APP on the We Chat platform. The credibility of APP embedding RF model for evaluating hardness and anthocyanin content in BHB was validated with the determination coefficient of 0.89 and 0.93 in practice. This approach could efficiently and conveniently evaluate the quality indexes of BHB in real time and serve as a technical reference for the detection of quality indicators of other berries using smartphones.
2026年01期 v.33;No.102 71-87页 [查看摘要][在线阅读][下载 2581K] [下载次数：5 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：1 ] - Liu Xinran;Wang Ying;Wei Zhiying;Ma Xinao;Shan Zhifu;Tang Lijie;
Coronavirus is an RNA virus that can infect both humans and animals, posing a significant threat to agriculture and public health. Although coronaviruses are highly host-specific, their ability to infect multiple hosts, combined with the structure of their genome, gives them a high probability of genetic recombination and mutation, leading to the creation of novel viruses. In recent years, with the establishment and development of reverse genetic manipulation techniques, substantial technical support has been provided for studying the structure and function of the coronavirus genome, the development of novel vaccines and drugs and the construction of viral expression vectors. This paper briefly described the progress in research on coronaviruses and their reverse genetic system construction strategies, aiming to provide some references for future coronavirus research.
2026年01期 v.33;No.102 88-96页 [查看摘要][在线阅读][下载 530K] [下载次数：3 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ] <正>Aims and Scope Journal of Northeast Agricultural University (English Edition) is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.
2026年01期 v.33;No.102 97页 [查看摘要][在线阅读][下载 337K] [下载次数：5 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：0 ]