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In soybean cotyledonary transformation,Agrobacterium infection is influenced by many factors.The cultivation stage of Agrobacterium strain and infectious concentration were studied in this research.Results showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =0.5.Seven soybean cultivars in North China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be suitable for transformation.This research has built a good foundation for soybean transformation improvement.
2010年02期 v.17;No.39 1-6页 [查看摘要][在线阅读][下载 281K] [下载次数：58 ] |[网刊下载次数：0 ] |[引用频次：4 ] |[阅读次数：40 ] A total of 104 isolates including two Korean isolates and three Japanese isolates of Phytophthora infestans collected from Heilongjiang and Jilin Provinces from 2006 to 2008 were used to determine their mating types,metalaxyl resistance,and RAPD genotypes.All the isolates of P.infestans collected from Heilongjiang and Jilin Provinces belonged to A 1 mating type,and no A 2 mating type was detected.Frequencies of metalaxyl resistant isolates were 94.4%,47.8% and 75.0% in 2006,2007 and 2008,respectively.According to RAPD analysis by using OPD-20 primer,104 isolates including two Korean isolates and three Japanese isolates were separated into 10 RAPD groups at 90.8% similarity level.A total of 10 RAPD groups were not correlated to groups defined by mating type,locations,and metalaxyl resistance.
2010年02期 v.17;No.39 7-14页 [查看摘要][在线阅读][下载 317K] [下载次数：59 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：38 ] -
Acetylcholinesterase (AChE) was immobilized on multilayer films assembled by poly diallyldimethylammonium chloride (PDDA) and ι-carrageenan (IC) on silver-coated crystal electrode surfaces to detect the chlorpyrifos belonging to the organophosphates pesticide.Mass sensitive quartz crystal microbalance (QCM) was used to study the effect of AChE concentration and pH of phosphate buffer solution on immobilized acetylcholinesterase.The optimized conditions were as follows: pH was 6.0 which was near isoelectric point (pI);enzyme concentration was 0.1 mg·mL -1;and the lowest concentration of chlorpyrifos that could be detected was 0.1 μg·L -1 .
2010年02期 v.17;No.39 15-21页 [查看摘要][在线阅读][下载 313K] [下载次数：40 ] |[网刊下载次数：0 ] |[引用频次：1 ] |[阅读次数：34 ] Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers' needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increase of EMS concentration,the mutagenic rate was increased,among which 1.0% EMS treatment had the biggest influence.However,with the same EMS concentration,there is no close relationship between the amount of mutagenic agent and the divergence of plants genomes,which offered a molecular basis for selecting plants with good mutation.
2010年02期 v.17;No.39 22-26页 [查看摘要][在线阅读][下载 295K] [下载次数：74 ] |[网刊下载次数：0 ] |[引用频次：6 ] |[阅读次数：52 ] -
Cre/LoxP site-specified recombination system is mainly used for excision,inversion and integration of target gene.Therefore,this system can be used for plant marker free genetic transformation,site-specific transgene expression and so on.However,the application of this system was limited due to its low expression and excision efficiency.In this study,an intron,which can enhance gene expression in plants,was inserted into Cre by using PCR method.And a modified Cre gene,named Crein,was obtained.This gene was composed of three parts: ATG,intron,Cre gene without ATG.This gene can be used for further research to provide the good system for plant marker free transformation.
2010年02期 v.17;No.39 27-31页 [查看摘要][在线阅读][下载 401K] [下载次数：55 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：39 ] One in 58 strains of bacteria isolated from the compost showed clear colonies after a few days of growth on the plates containing medium made of only agar and water.Water suspension contained only agar (2 and 8g·L -1 ) with two controls (normal saline,LB medium) was inoculated with the bacterium BR5-1 to see whether there was an increasement of the alive bacteria concentration after 48 h of the growth.The results showed that there was a significant rising of the alive bacteria concentration in the agar suspension (6.5×10 3 and 6.0×10 3 cfu·mL -1 ) compared to that in the normal saline control (6.1×10 2 cfu·mL -1 ),and it was far lower than that in LB (Luria-Bertani) control (1.1×10 8 cfu·mL -1 ).In conclusion,the bacterium strain BR5-1 could degrade agar.
2010年02期 v.17;No.39 32-35页 [查看摘要][在线阅读][下载 251K] [下载次数：37 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：47 ] -
The laws of water consumption in corn,peanuts and millet on the semi-drought area of western Liaoning Province were studied through the FAO-Penman Monteith method and the water balance method.Among three corps,the amount of the day water demand,the whole growth period water demand and the soil water deficit of corn were all the largest.At the same time,its degree of agreement between the water demand and the level of precipitation was the worst,and its average in crop coefficient was larger.The amount of the day water demand,the soil water deficit and its average in crop coefficient of millet were larger.Because of its short growth period,the whole millet growth period water demand was the least,resulting in its degree of agreement between the water demand and the level of precipitation was better.For peanuts,the amount of the day water demand,the soil water deficit and its average in crop coefficient were both the least,the whole growth period water demand was less,degree of agreement between the water demand and the level of precipitation was better,the average in crop coefficient was less,the change of crop coefficient was relatively steady,and water demand in the early and middle period of crop growth was less.From water use efficiency,crop plant order of regional priorities should be corn,millet,and peanuts.From economic benefits of water,crop plant order of regional priorities should be peanuts,corn,and millet;and crop plant order of priority to irrigation should be peanuts,millet,and corn.
2010年02期 v.17;No.39 36-42页 [查看摘要][在线阅读][下载 321K] [下载次数：53 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：51 ] - ZAKIR Shahidanasreen;
The concentration of heavy metals was investigated in black soil samples collected from the fields treated with chemical in Bayan County which were from the vegetation covers,bare soil and grain fields without any chemical treatments in Hailun National Field Research Station of Agroecosystem,Chinese Academy of Sciences (CAS) in July,2009.Results showed that the presence of significantly high concentration of zinc (Zn) and lead (Pb) in soils covered with grass vegetation.However,mercury (Hg),copper (Cu),nickel (Ni),cadmium (Cd),and arsenic (As) were found within the permissible limit set by international environmental agencies.
2010年02期 v.17;No.39 43-47页 [查看摘要][在线阅读][下载 349K] [下载次数：37 ] |[网刊下载次数：0 ] |[引用频次：2 ] |[阅读次数：68 ] -
Reasons for the color change of E.pulcherrima were studied with physiological and biochemical paraments such as: chlorophyll,carotenoids,anthocyanin,Phenylalanine ammonia-lyase (PAL),pH of cell sap in leaves and soluble sugar content etc.The results showed that during the period of the leaf color transmittion of E.pulcherrima,the contents of plastid pigment,soluble sugar and pH of cells sap in leaves showed a high-low-high dynamic change,while the contents of anthocyanin and PAL activity showed the low-high-low dynamic change.Anthocyanin stability had the close relationship with pH of cell sap in leaves,temperature and other conditions.
2010年02期 v.17;No.39 48-54页 [查看摘要][在线阅读][下载 304K] [下载次数：84 ] |[网刊下载次数：0 ] |[引用频次：4 ] |[阅读次数：35 ] In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were screened out from 20 primers and 47 DNA fragments were amplified from 39 individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and the polymorphic rate was 87.23%,which could reflect the genetic variation that was with four plots of each kind and to the individuals.Cluster results showed that at first,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were grouped together,and then they grouped with the grey A.chinense of green grassland into one cluster,at last with the grey-green A.chinense of green grassland.A.chinense of identical synusiologic type was grouped together.According to Shannon index,the green grassland of the grey A.chinense had the largest variation degree,which indicated that A.chinense had variation among species and interspecies by ISSR analysis,but A.chinense didn't differentiate different species.
2010年02期 v.17;No.39 55-59页 [查看摘要][在线阅读][下载 314K] [下载次数：44 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：40 ] -
The experiment was conducted to approve the efficacy of functional compound recipe liquid of eliminating lead and calcium to eliminate lead in rats.Total 120 rats were divided into six groups according to weight: blank group (CK),negative group (ND),positive group (PD) and high group (HD,6.67 Mg·kg -1·BW·d -1 ),middle group (MD,3.33 Mg·kg -1·BW·d -1 ) and low group (LD,1.67 Mg·kg -1·BW·d -1 three groups).Except CK,other groups were drunk 0.24% Pb(AC) 2 ,in which rats were filled with functional compound recipe liquid to eliminate lead and DSMA was added to positive group (PD).On the 20th day,the blood was collected from the tail.on the 40th day,the whole blood was collected from eyeball.After executed,liver and blood were collected to determine the content of lead and calcium.Results showed that lead contents in blood and liver of three dosage groups obviously decreased compared with ND and there was significant difference between any two groups of three dosage groups (P<0.01).Meanwhile,the calcium concentration increased in three dosage groups.It is concluded that the functional compound recipe liquid of eliminating lead has the evident function of eliminating lead and can promote calcium absorption.
2010年02期 v.17;No.39 60-63页 [查看摘要][在线阅读][下载 263K] [下载次数：30 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：42 ] Fuzzy comprehensive evaluation method was used to develop a standard model to analyze and evaluate nearness degree of water environment quality at breeding area of Shuidong Bay in China.Results showed that certain environment contamination factors in some areas seriously exceeded the standard value and led to the whole water quality at the third class level.The measurements should be taken to promote the sustainable development of breeding area in Shuidong Bay.
2010年02期 v.17;No.39 64-68页 [查看摘要][在线阅读][下载 286K] [下载次数：105 ] |[网刊下载次数：0 ] |[引用频次：3 ] |[阅读次数：43 ] -
With the globalization expanding,the coverage of the city grows the same increasingly.The situation that different streets and cities have almost the same scenes is becoming more and more serious.Creating regional characteristics of landscape is so urgent.Because only the local that is the national,and the national one belongs to the world.In the globalization process,the landscape construction contains both the "regional" integration and destruction,at the same time,it is a "regional" re-creation process.The social life goes further into globalization in the 21st century,the globalization process is not only economical globalization,political globalization,but cultural globalization.So is it to the landscape construction.Through the construction and design of Tianjin Qiaoyuan Park,this paper probed out the "regional" design way of park city landscape in Tianjin,even in the whole nation.
2010年02期 v.17;No.39 69-72页 [查看摘要][在线阅读][下载 208K] [下载次数：40 ] |[网刊下载次数：0 ] |[引用频次：1 ] |[阅读次数：35 ] The paper studied the competitive power of agricultural products in Heilongjiang Province by analyzing the revealed comparative advantage,and drew the conclution that agricultural products' competitive power was decreasing in Heilongjiang Province.There were three main reasons for this status: firstly,the cost of circulation of agricultural products was increasing;secondly,the commodity rate of agricultural products was low;thirdly,the marketing power of agricultural product was weak because of lacking the macro-management mechanism.To solve this problem,modern logistics must be developed to promote the competitive power of agricultural products in Heilongjiang Province.
2010年02期 v.17;No.39 73-77页 [查看摘要][在线阅读][下载 240K] [下载次数：218 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：147 ] -
The status of rural public goods' supply plays a decisive role in China agricultural development and in new socialist rural construction.For the current situation of insufficient supply of public goods in rural areas,this article explained the significance of strengthening the construction of rural public goods supply system,analyzed the reasons for the shortage of rural public goods supply,and proposed strategies and measures to solve problems of rural public goods supply,which are of advantages to enhance and improve the efficiency of rural public goods,having certain practical and theoretical values.
2010年02期 v.17;No.39 78-84页 [查看摘要][在线阅读][下载 203K] [下载次数：161 ] |[网刊下载次数：0 ] |[引用频次：1 ] |[阅读次数：126 ] Garlic virus infection is an important disease which affects garlic production,with the increasing years of planting,harm of virus is serious year by year,which seriously affect yield and quality of garlic.In order to know the garlic virus effectively,the paper reviewed the research situation of several important garlic virus in virus species,origin,distribution,host range,symptom,route of transmission,classification,genome and detection technique and the prevention technology of garlic viruses.At the same time,prospected several important research aspects of garlic virus in the future.
2010年02期 v.17;No.39 85-92页 [查看摘要][在线阅读][下载 230K] [下载次数：95 ] |[网刊下载次数：0 ] |[引用频次：2 ] |[阅读次数：22 ] -
Concept and application requirements of climate scenarios were introduced briefly,meanwhile,progresses on theoretical and applied aspects of climate scenarios creation techniques were discussed systematically.Two methods on predicted regional climate changing scenarios,elevating the spatial resolution output and downscaling method,could retrieve the insufficiencies respectively.And the statistical-dynamical downscaling method will be an important developing trend in the developing of downscaling techniques.
2010年02期 v.17;No.39 93-96页 [查看摘要][在线阅读][下载 195K] [下载次数：27 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：24 ] 下载本期数据