Journal of Northeast Agriultural University(English Edition)

2019, v.26;No.77(04) 63-72

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Isolation and Identification of Porcine Epidemic Diarrhea Virus(PEDV) HLJ Strain with IPEC-J2 Cells and Phylogenetic Analysis of Its S Gene
Isolation and Identification of Porcine Epidemic Diarrhea Virus(PEDV) HLJ Strain with IPEC-J2 Cells and Phylogenetic Analysis of Its S Gene

Feng Rui;Liu Hai-xin;Zhong Ming;Li Xun-liang;Huang Xiao-dan;Ren Yu-dong;Li Guang-xing;

摘要(Abstract):

Porcine epidemic diarrhea(PED) is caused by porcine epidemic diarrhea virus(PEDV), and is characterized by vomiting, diarrhea and dehydration of suckling pigs from 80% to 100% morbidity and 50% to 90% mortality, and resulted in tremendous economic losses to swine industry.The PEDV mainly infects small intestine of pigs, resulting in vacuolar degeneration and necrosis of mucosal epithelium.The IPEC-J2 is a pig intestine epithelial cell line, which is similar to the intestinal environment of piglets, can be used to isolate and identify the PEDV field isolates.In this study, it appeared the PEDV typical postmortem changes and histopathological lesion of degeneration and destruction of small intestine in infected piglets, and IHC identified that the PEDV distributed in the mucosa and submucosa of small intestine mostly.Furthermore, the PEDV HLJ strain was successfully isolated and characterized in the IPEC-J2 cells, and indicated that the IPEC-J2 cell line was sensitive to isolate and adapt the PEDV field strain, and could be utilized to multiply the PEDV rapidly.The S gene analysis indicated that the PEDV HLJ strain was the prevailed virus, belonged to Group 1 with attenuated virulent DR13, SC1402 and J-S2/2015 strains isolated in South Korea and China from 2014 to 2015.This study had important theoretical and practical significances on analyzing genetic variation of the PEDV, understanding the pathogenic characteristics of the virus and developing new vaccines for the PED.

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基金项目(Foundation): Supported by the National Natural Science Foundation of China(31201911; 31372438)

作者(Author): Feng Rui;Liu Hai-xin;Zhong Ming;Li Xun-liang;Huang Xiao-dan;Ren Yu-dong;Li Guang-xing;

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